FURTHER STUDIES ON ISOLATED CELL NUCLEI OF NORMAL RAT LIVER
نویسندگان
چکیده
منابع مشابه
The metabolism of isolated rat-liver nuclei.
The investigations described in this paper were undertaken as a preliminary to the study of the biochemical changes in the nucleus of the rat-liver cell during the induction of liver cancer. Allfrey (1954) and Stern & Mirsky (1953) described the isolation in aqueous media of nuclei from the thymus gland of the calf. Such preparations in vitro incorporated glycine and other amino acids into nucl...
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1. Parenchymal, Kupffer and biliary tract cells were isolated from normal rat liver by perfusion with collagenase solution. 2. The specific activities (munits of enzyme activity/mg of protein) of marker enzymes for the principal subcellular organelles were determined in the isolated cell homogenates and compared with whole liver homogenates. 3. The cells were disrupted and the extracts subjecte...
متن کاملStudies on Isolated Nuclei
This paper describes the subfractionation of nuclei isolated from guinea pig liver by the procedure presented in the first article of the series (8). Centrifugation in a density gradient system of nuclear fractions disrupted by sonication permits the isolation of the following subfractions: (a) a nucleolar subfraction which consists mainly of nucleoli surrounded by a variable amount of nucleolu...
متن کاملEnzymatic studies on isolated nucleoli of rat liver.
Nucleoli were prepared from rat liver nuclei isolated in 2.2 M sucrose. Assays for glutamate dehydrogenase, adenylate kinase, catalase, acid phosphatase, 5’-nucleotidase, glucose 6-phosphatase, lactate dehydrogenase, and pyruvate kinase as markers for cytoplasmic contamination revealed that the isolated nucleoli were in a high state of purity. Studies of the intranuclear distribution pattern of...
متن کاملEffect of ribosomal proteins on synthesis and assembly of preribosomal particles in isolated rat liver nuclei.
Ribosomal proteins are rapidly taken up by isolated rat liver nuclei. The proteins are localized mainly in the nucleolus and are found associated with a nucleolar 80 S particle containing newly synthesized 45 S RNA. Ribosomal proteins in the nucleoplasm were associated with particles containing 18 and 28 S RNAs. In the absence of ribosomal proteins in the incubations, there was a decrease in th...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1943
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)72132-7